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Dicer1 mutant mice exhibit visible phenotypes evocative of premature aging (A) The survival of a cohort of 62 wild-type (Dicer1 +/+ ) and 60 mutant (Dicer1 d/d) mice was followed for 80 weeks. (B) The occurrence of ulcerative dermatitis was quantified and expressed as a percentage (%) of mutant (60) and control (65) animals showing the phenotype. (C) Kyphosis in Dicer1 d/d mice (bottom) is shown by a red arrow. (D) Conjunctivitis in Dicer1 d/d mice (bottom). Control mice (Dicer1 +/+ ) are shown in the top pictures. (E) Weight (expressed in grams, g) was measured for 30 controls (Dicer1 +/+ ) and 30 mutants (Dicer1 d/d ) for 100 weeks. (F) Violin plot showing circulating (Plasma) <t>Leptin</t> (expressed in ng/ml) quantified by <t>ELISA</t> in 40 (N = 16) and 80 weeks-old (N = 13) control and Dicer1 d/d mice. (G) Violin plot showing the amount of adipose tissue (expressed as a percentage of the total body weight) quantified in 20-, 40- and 80-week-old control (Dicer1 +/+ ) and mutant (Dicer1 d/d ) mice following necropsy. In panels A and B, data were analyzed with the Log-rank test. In panels E, F and G, Data were analyzed by comparing two datasets with a Mann-Whitney U test. ∗p<0.05; ∗∗p<0.01; ∗∗∗p<0.001; ∗∗∗∗p<0.0001.Data are represented as mean ±SEM. See also <xref ref-type=Figure S1 and Table S1 . " width="250" height="auto" />
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Dicer1 mutant mice exhibit visible phenotypes evocative of premature aging (A) The survival of a cohort of 62 wild-type (Dicer1 +/+ ) and 60 mutant (Dicer1 d/d) mice was followed for 80 weeks. (B) The occurrence of ulcerative dermatitis was quantified and expressed as a percentage (%) of mutant (60) and control (65) animals showing the phenotype. (C) Kyphosis in Dicer1 d/d mice (bottom) is shown by a red arrow. (D) Conjunctivitis in Dicer1 d/d mice (bottom). Control mice (Dicer1 +/+ ) are shown in the top pictures. (E) Weight (expressed in grams, g) was measured for 30 controls (Dicer1 +/+ ) and 30 mutants (Dicer1 d/d ) for 100 weeks. (F) Violin plot showing circulating (Plasma) Leptin (expressed in ng/ml) quantified by ELISA in 40 (N = 16) and 80 weeks-old (N = 13) control and Dicer1 d/d mice. (G) Violin plot showing the amount of adipose tissue (expressed as a percentage of the total body weight) quantified in 20-, 40- and 80-week-old control (Dicer1 +/+ ) and mutant (Dicer1 d/d ) mice following necropsy. In panels A and B, data were analyzed with the Log-rank test. In panels E, F and G, Data were analyzed by comparing two datasets with a Mann-Whitney U test. ∗p<0.05; ∗∗p<0.01; ∗∗∗p<0.001; ∗∗∗∗p<0.0001.Data are represented as mean ±SEM. See also <xref ref-type=Figure S1 and Table S1 . " width="100%" height="100%">

Journal: iScience

Article Title: Dicer1 deficient mice exhibit premature aging and metabolic perturbations in adipocytes

doi: 10.1016/j.isci.2022.105149

Figure Lengend Snippet: Dicer1 mutant mice exhibit visible phenotypes evocative of premature aging (A) The survival of a cohort of 62 wild-type (Dicer1 +/+ ) and 60 mutant (Dicer1 d/d) mice was followed for 80 weeks. (B) The occurrence of ulcerative dermatitis was quantified and expressed as a percentage (%) of mutant (60) and control (65) animals showing the phenotype. (C) Kyphosis in Dicer1 d/d mice (bottom) is shown by a red arrow. (D) Conjunctivitis in Dicer1 d/d mice (bottom). Control mice (Dicer1 +/+ ) are shown in the top pictures. (E) Weight (expressed in grams, g) was measured for 30 controls (Dicer1 +/+ ) and 30 mutants (Dicer1 d/d ) for 100 weeks. (F) Violin plot showing circulating (Plasma) Leptin (expressed in ng/ml) quantified by ELISA in 40 (N = 16) and 80 weeks-old (N = 13) control and Dicer1 d/d mice. (G) Violin plot showing the amount of adipose tissue (expressed as a percentage of the total body weight) quantified in 20-, 40- and 80-week-old control (Dicer1 +/+ ) and mutant (Dicer1 d/d ) mice following necropsy. In panels A and B, data were analyzed with the Log-rank test. In panels E, F and G, Data were analyzed by comparing two datasets with a Mann-Whitney U test. ∗p<0.05; ∗∗p<0.01; ∗∗∗p<0.001; ∗∗∗∗p<0.0001.Data are represented as mean ±SEM. See also Figure S1 and Table S1 .

Article Snippet: Leptin and Insulin concentration were estimated by ELISA following the manufactures’ guidelines (Mouse/Rat Leptin Quantikine ELISA Kit, R&D Systems; Rat/Mouse Insulin ELISA kit, Merck Millipore).

Techniques: Mutagenesis, Control, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: iScience

Article Title: Dicer1 deficient mice exhibit premature aging and metabolic perturbations in adipocytes

doi: 10.1016/j.isci.2022.105149

Figure Lengend Snippet:

Article Snippet: Leptin and Insulin concentration were estimated by ELISA following the manufactures’ guidelines (Mouse/Rat Leptin Quantikine ELISA Kit, R&D Systems; Rat/Mouse Insulin ELISA kit, Merck Millipore).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, SYBR Green Assay, Software